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Liquid Chromatography of Synthetic Polymers
Scientific
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ForAbstractSubmission:
Dusan
Berek, Prof, PhD, DSc Employed at Polymer Institute, Slovak Academy of Sciences
in Bratislava. Served as elected member of the Presidium of the Slovak Academy
of Sciences, President of the Slovak Chemical Society, Chairman of the
Czecho-Slovak and Slovak National Committee of Chemistry for IUPAC.
Corresponding member of the Central European Academy of Sciences and member of
the Learned Society of the Slovak Academy of Sciences. Author or co-author of
two monographs and 300+ scientific papers in extenso published in refereed
periodicals, proceedings and chapters of books, as well as 60+ patents (four of
them were licensed) - cited more than 3,000x. Presented over 130 invited
plenary, key and main lectures, as well as over 900 regular lectures and poster
contributions on symposia and conferences, as well as during lecturing tours to
over fourty countries. Elected „Slovak scientist of the year 1999“, and „Slovak
innovator of the year 2002“
High
performance liquid chromatographic (HPLC) methods represent an important
practical tool for molecular characterization of synthetic polymers. Mean molar
mass (MM) and molar mass distribution (MMD) of linear and branched homopolymers
can be readily determined by gel permeation (size exclusion) chromatography
(GPC/SEC). GPC/SEC also provides several other useful data on polymers such as
limiting viscosity numbers, constants of viscosity law, sizes of macromolecules
in solution - and even on extent of their preferential solvation in mixed
solvents. Recent progress in GPC/SEC comprises improved instrumental hardware
and data processing procedures. The ultra-high pressure GPC/SEC enables
significant acceleration of analyses, which is especially important in
combinatorial material chemistry and in polymer production control. Still,
further improvements of the GPC/SEC method are needed comprising its hardware,
especially columns and detectors, as well as standardization of sample
preparation, measurement, and data processing. GPC/SEC exhibits excellent
intra-laboratory repeatability, which evokes a notion of its high reliability.
However, recent series of the round robin tests revealed surprisingly poor
inter-laboratory reproducibility of results. Evidently, accuracy of many
GPC/SEC results may be rather limited. Moreover, GPC/SEC hardly enables precise
molecular characterization of complex polymer systems, which possess more than
one distribution in their molecular characteristics. Typically, polymer
mixtures, copolymers and functional polymers exhibit besides MMD also
distribution in their chemical structure. To assess above distributions, new
HPLC procedures are developed. These make use the controlled combinations of
entropic (exclusion) and enthalpic (interaction) retention mechanisms within
one column or in a series of independent separation systems. Enthalpic
retention mechanisms in HPLC of synthetic polymers include adsorption,
partition and phase separation. The resulting approaches are denoted “coupled
polymer HPLC” and “two-dimensional polymer HPLC”. We shall review recent
progress and problems in GPC/SEC, as well as in coupled, and two-dimensional
polymer HPLC procedures, and outline anticipated future development.
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